Ca-Sensitive Fluorescent Dyes and Intracellular Ca Imaging
نویسندگان
چکیده
Martin D. Bootman,1,2,5 Katja Rietdorf,3 Tony Collins,4 Simon Walker,1 and Michael Sanderson3 Babraham Institute, Babraham, Cambridge, CB22 3AT, United Kingdom; Department of Life, Health and Chemical Sciences, The Open University, Walton Hall, Milton Keynes, MK7 6AA, United Kingdom; Department of Microbiology and Physiology Systems, University of Massachusetts Medical School, Worcester, Massachusetts 01655; McMaster Stem Cell and Cancer Research Institute, Faculty of Health Sciences, McMaster University, MDCL 5029, Hamilton, Ontario L8S4L8, Canada
منابع مشابه
Calcium mobilization by nicotinic acid adenine dinucleotide phosphate (NAADP) in rat astrocytes.
Nicotinic acid adenine dinucleotide phosphate (NAADP) has been shown to release intracellular Ca(2+) in several types of cells. We have used Ca(2+)-sensitive fluorescent dyes (Fura-2, Fluo-4) to measure intracellular Ca(2+) in astrocytes in culture and in situ. Bath-applied NAADP elicited a reversible and concentration-dependent Ca(2+) rise in up to 90% of astrocytes in culture (EC(50)=7 microM...
متن کاملEstablishment of a New Cell-Based Assay To Measure the Activity of Sweeteners in Fluorescent Food Extracts
Taste receptors have been defined at the molecular level in the past decade, and cell-based assays have been developed using cultured cells heterologously expressing these receptors. The most popular approach to detecting the cellular response to a tastant is to measure changes in intracellular Ca(2+) concentration using Ca(2+)-sensitive fluorescent dyes. However, this method cannot be applied ...
متن کاملDetermining zinc with commonly used calcium and zinc fluorescent indicators, a question on calcium signals.
Investigations into the roles of Ca(2+) and Zn(2+) in cell biology have been facilitated by the development of sensitive fluorometric probes that have enabled the measurement of Ca(2+) or Zn(2+) in both extracellular and intracellular environments. It is critical to be aware of the specificity and relative selectivity of a probe for the targeted ion. Here, we investigated metal-ion responses by...
متن کاملCytotoxic action of triphenyltin on mouse thymocytes: a flow-cytometric study using fluorescent dyes for membrane potential and intracellular Ca2+.
Effects of triphenyltin on mouse thymocytes were examined using fluorescent dyes to monitor membrane potential and intracellular Ca2+. Triphenyltin at 3 x 10(-7) M to 1 x 10(-6) M hyperpolarized thymocytes and depolarized them at 3 x 10(-6) M or more, associated with increasing intracellular Ca2+. Hyperpolarization was suppressed by quinine, but not by tetraethylammonium and 4-aminopyridine, su...
متن کاملCell alkalosis elevates cytosolic Ca2+ in rabbit resident alveolar macrophages.
Disruption of cellular acid-base status alters the host defence functions of alveolar macrophages (m phi). These pH effects might be mediated by pH-sensitive changes in the signalling pathways of the effector functions of m phi. The present study examined the effects of intracellular pH (pH(i)) on the free cytosolic calcium concentration ([Ca(2+)](i)), an important second messenger for cell fun...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2013